ImFCS Software

ImFCS is a software to perform auto- and cross-correlations of image stacks acquired from camera based FCS. The software is programmed to work in Windows © OS with Igor Pro © (Ver 6.0 and later) as front end. This software can read image stacks, calculate correlations, fit the curves and display the parameters as images.


Latest Update (updated on Apr 14 2016)

The "numer of particles" image displayed after fitting needs to be multiplied by a factor 4 to accurately show the number of particles per pixel.

Installation files (updated on Oct 1 2014)

The xop and ipf files can be downloaded here.

Previous Updates

Oct 01 2014: The program is compatible with Igor Pro ver 6.2

Jan 26 2011: user can provide an option to calculate the point upto which the area under the curve needs to be evaluated for delta CCF and delta CCF histogram buttons added

The manual can be downloaded here.

The videos can be downloaded here .

The source VC++ .net 2003 codes can be downloaded here.

The supplementary material for "ImFCS: A software for Imaging FCS data analysis and visualization" by Sankaran et al (Optics Express 2010, Vol. 18, Iss. 25, pp. 25468 - 25481) is attached here.

A trial data set along with the autocorrelations can be downloaded here.


1. There have been instances of the program crashing when used in Windows 7 64 bit. Hence it is advised to avoid this particular OS configuration.

2. ImFCS at the moment runs only under IgorPro 6.22A but not under the newer version 6.3.

3. The "kill" button can only be used for a limited number of times (4 to 10) depending on the file size loaded due to a memory mismanagment problem.


The software and data on this site are provided for personal or academic use only and may not be used in any commercial venture or distributions. All files have been virus scanned, however, for your own protection; you should scan these files again. You assume the entire risk related to your use of this software and data. By using the software and data on this site your expressly assume all risks of data loss or damage alleged to have been caused by the software and data. The Biophysical Fluorescence Laboratory at NUS is providing this data "as is," and disclaims any and all warranties, whether express or implied, including (without limitation) any implied warranties of merchantability or fitness for a particular purpose. In no event will the Biophysical Fluorescence Laboratory at NUS and/or NUS be liable to you or to any third party for any direct, indirect, incidental, consequential, special or exemplary damages or lost profit resulting from any use or misuse of this software and data.

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  • Biophysical Fluorescence Laboratory