Ph.D. (Biological Sciences)
National University of Singapore, Singapore
B.Tech. (Industrial Biotechnology)
Anna University, India
Signalling proteins toggle between multiple conformations (conformational dynamics) to carry out their functions. My work aims to probe the conformational dynamics and allosteric relays in proteins populating transient transition states using structural mass spectrometry, specifically amide hydrogen/deuterium exchange mass spectrometry (HDXMS). I am mainly interested in correlating structure and function through an understanding of protein dynamics. To this end I study protein dynamics in multiple systems, ranging from signaling proteins in the cAMP signaling pathway, protein-ligand interactions, virus maturation pathways and protein-DNA interactions.
B.Sc. (Hons.) (Biotechnology)
Universiti Tunku Abdul Rahman (UTAR), Malaysia
Membrane proteins are integral sensors of changes in the extracellular environment. How extracellular signals are sensed and transduced to elicit intracellular response(s) is still a biological mystery. I am interested in the conformational dynamics of membrane proteins during the signal transduction process. Amide hydrogen/deuterium exchange mass spectrometry (HDXMS) is a very robust platform to probe the dynamics of proteins when they are perturbed by different conditions and is the major technique used in my experiments. Research on a membrane-bound bacterial histidine kinase that senses changes in environmental osmolality has provided important insights into the mechanisms of signal transduction and how it can be applied for other membrane proteins.
Ph.D. (Computational Systems Biology)
National University of Singapore
M.Sc. (Biotechnology) and B.Sc (Biochemistry)
University of Madras, India
I work on computational modelling of protein dynamics based on biophysical data from HDX. Combining protein dynamics data from hydrogen/deuterium exchange and molecular dynamics simulations, I aim to describe the atomistic mechanisms of protein signalling.
Indian Institute of Technology, Guwahati, India
University of Calcutta, India
Signal transmission across cell membranes is critical for cellular function but is a poorly understood phenomenon. My project interests are focussed on exploring mechanisms of transmembrane signal transduction. I’m currently working on bacterial membrane proteins which are involved in sensing and responding to changes in environmental osmolality. I’m also interested in understanding how mammalian cell surface receptors respond to hormonal stimulation and elucidate a cytoplasmic response.