Protein and Proteomics Centre

A CENTRE FOR RESEARCH ON PROTEINS, PEPTIDES AND FUNCTIONAL GENOMICS

The Protein and Proteomics (PPC) is a multi-user core facility located at Block S2-Level 2, focused on advanced research in proteins with an emphasis on mass spectrometry. Areas of expertise include biomarker discovery, proteomics, quantitative proteomics, and structural mass spectrometry (Amide Hydrogen/Deuterium Exchange MS and Ion Mobility MS). PPC works closely with academic and industrial partners in biomedical research.

Our Mission

Our goal is to provide state-of-the-art mass spectrometry services and expertise while expanding MS applications across the Life Sciences spanning biology, chemistry and pharmacy. We aim to build on our status as a Waters World Centre of Innovation in HDXMS and a SCIEX Regional Centre of Distinction to other areas of mass spectrometry applications through partnerships with individual researchers and industrial collaborations.

Mass spectrometers

LCMS-TripleTOF 5600 System (AB Sciex);

Synapt G1 High Definition Mass Spectrometer (HDMS, Waters);

Synapt G2Si High Definition Mass Spectrometer (HDMS, Waters)

 

Staff

http://www.dbs.nus.edu.sg/ppc/images/coi_anand_01.jpgGanesh Srinivasan Anand

Director

Associate Professor, Department of Biological Sciences

 

 

 

 

http://www.dbs.nus.edu.sg/ppc/images/linqs(2).jpgLin Qingsong

Co-Director

Senior Research Fellow, Department of Biological Sciences

 

 

http://www.dbs.nus.edu.sg/images/staff/lab%20(32).jpgWang Xianhui

Lab Technologist

65164594

dbswxh@nus.edu.sg

 

http://www.dbs.nus.edu.sg/images/staff/teckkwang.jpgLim Teck Kwang

Senior Scientific Officer

65164594

dbslimtk@nus.edu.sg

 

 

CORE SERVICES

1.   In-gel Protein Identification

Identification of protein from gel bands/spots by MS

 

2.   Shotgun Protein Identification

Complex protein mixture (e.g. co-IP/pull-down sample)

 

3.   Molecular Weight Determination

Determining molecular weight of intact protein in solution by MS

 

4.   Post-Translational Modifications

Methods to study post-translational modifications such as sumoylation etc.

 

 

COLLABORATIVE SERVICES

1.   Phosphoproteomics

Protein identification and phosphorylation analysis

 

2.   Protein Structure, Dynamics and Interactions

Amide hydrogen/deuterium exchange mass spectrometry (HDXMS)

 

3.   Quantitative Proteomics

iTRAQ - labelling coupled to LC-MS/MS / SWATH/ MRM /MRM-HR

 

 

Expertise Areas:

 

Structural Mass Spectrometry

A/P Ganesh Anand

 

Quantitative Proteomics, Phosphoproteomics, Biomarker discovery

Dr Lin Qingsong

 

Mass Spectrometry in-charge

 

Ms Wang Xianhui; Mr Lim Teck Kwang

 

User fees:

 

 

Sample submission form (please click)


Location / Contact

Protein and Proteomics Centre, PPC

S2 - Level 2, Department of Biological Sciences

NUS, Singapore 117543

Tel: 65164594 / 65167754 (through Wang Xianhui / Lim Teck Kwang)

Email: dbsbox32@nus.edu.sg

 

Note: A comprehensive website is under construction.