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ABI 3100 XL Operator's Guide
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PART
A
Guidelines
for the Users Who Perform Their Own Runs
I.
Booking
- Sequencers
available:
- Two
16-capillary sequencers (ABI 3130xl Genetic Analyzer),
labeled 3130A & 3130B
- One
96-capillary sequencers (ABI 3730xl Genetic
Analyzer), labeled 96-Cap
3131A & B |
96-Cap |
16
slots/day |
12
slots/day |
1
slot = 1.5-hr run = 16 samples |
1
slot = 2hr run = 96 samples
Note:
book this sequencer if you have 48 samples or more |
3.
Authorized users:
- Principal
investigators (PIs), graduate students or staff of Department
of Biological Sciences [undergraduate
(including Honours) students are not allowed to make booking
for user runs].
- Each
user must have an associated PI laboratory.
- LSM
module coordinator requiring the use of DSL facility to kindly
liaise with A/P Sanjay Swarup at the start of the semester.
4.
Booking periods:
- Users
may start booking at a maximum of 14 days in advance.
- Changes
may be freely made as long as slots are available. However,
cancellation is not allowed on the day of run.
- You
will be given PENALTY/WARNING points & fine
[$27.20/slot (3130A & B), $81.60/slot (96 Cap)] if
you fail to use your booked slots.
5.
Priority & usage limits:
3130A & B
(Cater for 'light' users) |
96-Cap
(Cater for users doing large scale sequencing & fragment
analysis) |
First-come-first-served
basis |
Simultaneous
booking of 3 sequencers on the same day from the same PI
lab is not allowed
Note:
this rule does not apply to vacant slots 1 day before & on
the day of run |
MAXIMUM
4 slots/day
Note:
this limit does not apply to vacant slots 1 day before & on
the day of run |
No
usage limit per week |
These
rules are subject to change depending on the usage patterns.
II.
Operating the sequencer
- Only
trained staff and postgraduate students registered with
DSL are allowed to operate the sequencer. Other users will
have to be trained by their respective laboratory representatives.
DSL staff will only train representatives of laboratories
where previously no member was trained. The names of these
(new) operators will have to be registered with
us first prior to their use of the equipment. NOTE:
Honours and UROPS students are not allowed to operate the
sequencer.
- Purchase
your own 96 well plate (ABI, Biogen or Research Instrument)
and HI DI formamide (25ml) ABI, [Part no. 4311320]. Other
consumables will be provided by DSL.
III.
Data retrieval, analysis and printing
- Data
downloading, sequence viewing & analysis: Users
are to download their data from the computers designated
for data analysis & downloading.
- Printing:Users
are to print their data in their own labs. The software required:
Software
from ABI: Sequence
Scanner Software v1.0
Chromas
Version 1.43: http://www.technelysium.com.au/chromas.html
4Peaks
(Mac Users): http://mekentosj.com/4peaks/
- File
deletion: Files
will be retained in the DSL computer for a maximum period
of one month, after which they will be deleted by DSL staff
without warning. Users are not allowed to delete any
files/run folders from the hard disk. Deleted files/run
folders will be taken as cancelled/no show and PENALTY/WARNING will
be issued.
IV.
Billing
1.
Cost:
3131A & B |
96-Cap |
Flat-rate
of $27.20/slot
1
slot =16 samples
|
more
than 48
samples: $81.60
49-64
samples: $108.80
65-80
samples: $136.00
80-96
samples: $163.20 |
Cost/sample:
$1.70 |
2.
Periodic billing will be sent to the PIs. They will be given a week
to clarify/seek changes. The billing will have to be signed by PI
and returned to DSL staff. |
