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Ph.D. ( Rutgers University, USA), M. Sc.(hons.), B.Pharm. (hons.) (BITS Pilani, India)

MAJOR RESEARCH INTERESTS

Regulatory molecules in cellular signalling pathways serve as molecular switches that tightly regulate the activity of associated enzymes. Defining the interactions of regulatory switches with their target enzymes as well as role of posttranslational modifications, is key to understanding the molecular basis of cell signalling. Our research focuses on unraveling specific protein-protein and protein-ligand interactions in signaling enzymes. We are focusing our efforts on the various elements of the second messenger, adenosine 3', 5' cyclic monophosphate (cAMP) signalling pathway, in particular cAMP-dependent protein kinase (Protein Kinase A) and cAMP phosphodiesterases. We are also interested in studying phosphorylation and ligand-dependent conformational changes at protein interfaces. Research areas include:

• Molecular recognition and protein-protein interactions
• Phosphorylation and ligand-dependent regulation of signalling proteins
  

SELECTED PUBLICATIONS

• Ganesh S. Anand, Matthew Hotchko, Simon Brown, Lynn Ten Eyck, Elizabeth Komives and Susan S. Taylor. (2007). R-subunit Isoform Specificity in Protein Kinase A: Distinct Features of Protein Interfaces in PKA Types I and II by Amide H/2H Exchange Mass Spectrometry J. Mol. Biol. (in press).

• Ganesh S. Anand, Susan S. Taylor and David A. Johnson (2007). Cyclic-AMP and Pseudosubstrate Effects on A-Kinase Regulatory and Catalytic Subunit Binding Kinetics Biochemistry 46:9283-91.

• Matthew Hotchko, Ganesh S. Anand, Elizabeth A. Komives and Lynn F. Ten Eyck (2006). Automated Extraction of Backbone Deuteration Levels From Amide H/²H Mass Spectrometry. Protein Science 15(3):583-601.

• Yoshitomo Hamuro*, Ganesh S. Anand*, Jack S. Kim, Celina Juliano, David Stranz, Susan S. Taylor and Virgil Woods Jr. (2004). Mapping Intersubunit Interactions of the Regulatory Subunit (R1alpha) in the Type I Holoenzyme of PKA by Enhanced Amide Hydrogen/ Deuterium Exchange Mass Spectrometry (DXMS). J. Mol. Biol. 340(5):1185-96. 

• Ganesh S. Anand, Dennis Law, Jeffrey Mandell, Aaron Snead, Igor Tsigelny, Susan S. Taylor, Lynn Ten Eyck and Elizabeth A. Komives (2003). Structure of the Protein Kinase A Regulatory-Catalytic Subunit Complex by Amide H/²H Exchange and Protein Docking. Proc. Natl. Acad. Sci.(USA) 100: 13264-13269.

• Ganesh S. Anand, Carrie A. Hughes, John M. Jones, Susan S. Taylor and Elizabeth A. Komives (2002) Amide H/²H Exchange Reveals Communication Between the cAMP- and Catalytic Subunit-Binding Sites in Protein Kinase A. J. Mol. Biol. 323(2):377-86.

• Ganesh S. Anand and Ann M. Stock (2002).The Kinetic Basis for the Stimulatory Effect of Phosphorylation on the Methylesterase Activity of CheB Biochemistry v41: 6752-6760.

• Ganesh S. Anand, Paul Goudreau, J. Kathleen Lewis and Ann M. Stock (2000). Evidence for phosphorylation-dependent conformational changes in methylesterase CheB. Protein Science, 8(4): 153-156.