Ganesh Srinivasan ANAND
Ph.D. ( Rutgers University,
USA), M. Sc.(hons.), B.Pharm. (hons.) (BITS Pilani,
MAJOR RESEARCH INTERESTS
Our research involves unraveling specific protein-protein, protein-ligand and protein-nucleic acid interactions. One of the areas of focus is the cyclic adenosine monophosphate (cAMP) signaling pathway and we are studying two classes of enzymes, cAMP-dependent protein kinase (Protein Kinase A) and phosphodiesterases. Other areas of interest include protein-nucleic acid interactions, intrinsically disordered proteins and development of small molecule inhibitors for drug discovery using structural mass spectrometry tools including Amide Hydrogen/Deuterium (H/D) Exchange Mass Spectrometry and Ion Mobility Mass Spectrometry to complement X-ray crystallography. The overall goal of our research is to map phosphorylation and ligand-dependent conformational changes at protein interfaces using multidisciplinary approaches integrating biophysics and structural biology with biochemistry.
Wang Loo Chien, Jeremy, Leslie Morgan, Pahan Godakumbura, Linda J. Kenney and Ganesh S. Anand (2012) The Inner Membrane Histidine Kinase EnvZ Senses Osmolality via Helix-Coil Transitions in the Cytoplasm EMBO J. (in press).
Shubhlakshmi Nambi, Suguna Badireddy, Sandhya Visweswariah and Ganesh S. Anand (2012) Cyclic AMP-induced conformational changes in mycobacterial protein acetyltransferases J. Biol. Chem. (in press)
Moorthy, B.S. and Ganesh S. Anand (2012) Multi-State Allostery in Response Regulators: Phosphorylation and Mutagenesis Activate RegA via Alternate Modes J. Mol. Biol. 417(5):468-87
Suguna Badireddy, Gao Yunfeng, Jian Wu, Choel Kim, Susan S. Taylor, Lin Qingsong, Swaminathan, K. and Ganesh S. Anand (2011) Combination of Conformational Selection and Induced Fit in cAMP-mediated Allostery. Mol. Cell. Prot. 10(3):M110.004390
Balakrishnan Shenbaga Moorthy, Gao Yunfeng and Ganesh S. Anand (2011) Phosphodiesterases Catalyze Hydrolysis of cAMP bound to Regulatory Subunit of Protein Kinase A and Mediate Signal Termination. Mol. Cell. Prot. Feb;10(2):M110.002295
Balakrishnan Shenbaga Moorthy, Suguna Badireddy and Ganesh S. Anand (2011) Cooperativity and allostery in cAMP –dependent activation of Protein Kinase A: Monitoring conformations of intermediates by amide hydrogen/deuterium exchange . Intl J. Mass Spec. Volume 302, Issues 1-3, 30 April 2011, Pages 157-166.
Ganesh S. Anand, Srinath Krishnamurthy, Tanushree Bishnoi, Alexandr Kornev, Susan S. Taylor and David A. Johnson (2010) Cyclic-AMP and Rp-cAMPS Induced Conformational Changes in a Complex of the Catalytic and Regulatory (RIα) Subunits of PKA. Mol. Cell. Prot.2010 Oct;9 (10): 2225-37
Waters Center of Innovation in “Hydrogen/Deuterium Exchange Mass Spectrometry”: