PhD (Univ. of Pennsylvania)
Tumor angiogenesis, anti-angiogenic proteins and cancer therapeutics:
Angiogenesis (new blood vessel formation) is essential for tumor growth and metastasis. Anti-angiogenesis is a promising anticancer therapeutic approach. We focus on investigating novel endogenous angiogenesis inhibitor proteins that contain the thrombospondin type 1 repeat domain (TSR) and their function and development into anti-angiogenic cancer drugs. These proteins are not only valuable for anticancer drug development, but also important for maintaining angiogenesis homeostasis in normal tissues. We have identified 3 novel endogenous anti-angiogenic proteins: Isthmin, ADAMTS5 and ADAMTS4. We are currently investigating their pathophysiological function and therapeutic applications. In addition, we are studying tumor endothelial cell biology and developing targeted drug delivery for liver cancer using tumor endothelial cell surface markers.
Angiogenesis and liver organogenesis in zebrafish model:
Angiogenesis is very important for cardiovascular biology and medicine. It is essential for all tissue formation and growth. We use zebrafish as a model organism to study angiogenesis and liver development. Genes of interest include Augmenter of Liver Regeneration (ALR), Hepatocyte Growth Factor (HGF) and histone deacetylases (HDACs).
Kumar, S., S. Sharghi-Namini, N. Rao and R. Ge. ADAMTS5 functions as an anti-angiogenic and
anti-tumorigenic protein independent of its proteoglycanase activity. Am. J. Pathol. 181(3):1056-68, 2012. PubMed link
Ge R, Tan E, Sharghi-Namini S, Asada HH. Exosomes in cancer microenvironment and beyond: have we overlooked these extracellular messengers? Cancer Microenviron. 2012 May 15. PubMed link
Li Y, Farooq M, Sheng D, Chandramouli C, Lan T, Mahajan NK, Kini RM, Hong Y, Lisowsky T, Ge R. Augmenter of liver regeneration (alr) promotes liver outgrowth during zebrafish hepatogenesis. PLoS One 7(1):e30835, 2012. PubMed link
Zhang Y, Chen M, Venugopal S, Zhou Y, Xiang W, Li YH, Lin Q, Kini RM, Chong YS, Ge R. Isthmin exerts pro-survival and death-promoting effect on endothelial cells through alphavbeta5 integrin depending on its physical state. Cell Death Dis. 2:e153, 2011. PubMed link
Xiang W, Ke Z, Zhang Y, Cheng GH, Irwan ID, Sulochana KN, Potturi P, Wang Z, Yang H, Wang J, Zhuo L, Kini RM, Ge R. Isthmin is a novel secreted angiogenesis inhibitor that inhibits tumor growth in mice. J. Cell. Mol. Med. 15(2):359-74., 2011. PubMed link
Jia, J., J. Wang, M. Teh, W. Sun, J. Zhang, I. Kee, P. K-H Chow, R. C. M-Y. Liang, M. C.M. Chung and R. Ge. Identification Of Proteins Differentially Expressed Between Capillary Endothelial Cells Of Hepatocellular Carcinoma And Normal Liver In An Orthotopic Rat Tumor Model Using 2D-DIGE. Proteomics 10(2):224-34, 2010. PubMed link
Raghunath M, Sy Wong Y, Farooq M, Ge R. Pharmacologically induced angiogenesis in transgenic zebrafish. Biochem. Biophy. Res. Comm. 378:766-71, 2009. PubMed link
Yuan P, Bartlam M, Lou Z, Chen S, Zhou J, He X, Lv Z, Ge R, Li X, Deng T, Fodor E, Rao Z, Liu Y. Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site. Nature 458:909-13, 2009. PubMed link
Fan, H., K.N. Sulochana, Y-S Chong and R. Ge. Decorin Derived Antiangiogenic Peptide LRR5 Inhibits Endothelial Cell Migration By Interfering With VEGF-Stimulated NO Release. Int. J. Biochem. Cell Biol. 40, 2120-2128, 2008. PubMed link
Sharghi-Namini, S., H. Fan, K.N. Sulochana, P. Potturi, W. Xiang, Y-S Chong, Z. Wang, H. Yang, R. Ge. The first but not the second thrombospondin type 1 repeat of ADAMTS5 functions as an angiogenesis inhibitor. Biochem. Biophy. Res. Comm. 371:215-9, 2008. PubMed link
Farooq, M., K.N. Sulochana, X. Pan, J. To, D. Sheng, Z. Gong, R. Ge. Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish. Dev. Biol., 317:336-53, 2008. PubMed link
He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A, Zeng Z, Ge R, Rao Z, Liu Y. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature 454:1123-6, 2008. PubMed link
Sulochana, K. N and R. Ge. Developing Antiangiogenic Peptide Drugs for Angiogenesis-Related Diseases. Curr. Pharma. Des., 13:2074-86, 2007. PubMed link
Ye, L, KhH Haider, RS Tan, WC Toh, PK Law, WB Tan, LP Su, W Zhang, R Ge, Y Zhang, Y T Lim and E K W Sim, "Transplantation of nanoparticle transfected skeletal myoblasts overexpressing vascular endothelial growth factor 165 for cardiac repair". Circulation 116:I113-20, 2007. PubMed link
Sulochana, KN, H. Fan, S. Jois, S. Vivekanandan, F. Sun, R. M. Kini, and R. Ge. Peptides Derived From Human Decorin Leucine Rich Repeat 5 Inhibit Angiogenesis.
J. Biol. Chem. 280: 27935-27948, 2005. PubMed link
Sun, F., D. Casse, J. A. Van Kan, R. Ge* and F. Watt. Geometric Control of fibroblasts Growth on Proton Beam Micromachined Scaffolds. Tissue Eng. 10, 267-272, 2004. *Corresponding author. PubMed link
Shim, W. S. N, M. Teh, A. Bapna, I, Kim, G-Y Koh, P. Mack and R. Ge. Angiopoietin-1 Promotes Tumor Angiogenesis and Tumor Vessel Plasticity ofHuman Cervical Cancer in Mice. Exp. Cell Res. 279, 299-309, 2002. PubMed link
Liang, D., J. Chang, A. J. Chin, A. Smith, C. Kelly, Eric S. Weinberg and R. Ge. The Role of Vascular Endothelial Growth Factor (VEGF) in Vasculogenesis, Angiogenesis, and Hematopoiesis in Zebrafish Development. Mech. Dev. 108, 29-43, 2001. PubMed link
Tan, D C-W., M. R. Kini., J. D. S. Seetharama, D. K-F Lim, L. Xin and R. Ge. A Small Peptide Derived From Flt-1 (VEGFR-1) Functions As An Angiogenic Inhibitor. FEBS Lett. 494,150-156, 2001. PubMed link
Ge, R and R. M. Kini, Small Peptides With Potent Anti-Angiogenic Activities. US patent No. 7,317,003, 2008.
Ge, R and R. M. Kini, Small Peptides with Potent Anti-Angiogenic Activities. US patent No. 6,200,954, 2001.
updated 15 Feb 2013