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Wu Jinlu Lecturer Contact Information: Department of Biological Sciences National University of Singapore Science Drive 4, Singapore 117543 Tel: 65161687(lab), 65168476 (office) Email: dbswjl@nus.edu.sg

Teaching Areas

Molecular Genetics (LSM1102)
General Biology (LSM1301)
Experimental Molecular and Cell Biology (LSM2202)

Research Areas

Virology, Functional Genomics, Lipidomics

Research Interests

We are generally interested in aquatic viruses and viral diseases. With access to increasing sequences of virus genomes, we are now intensifying our efforts to elucidate viral protein structures and functions of two DNA viruses: white spot syndrome virus (WSSV) and Singapore grouper iridovirus (SGIV). Using a high-throughput proteomics approach, more than 50 proteins have been identified from the two viruses, but most of them are functionally unknown. We have set up several platforms such as structural biology, DNA/protein array and gene knockdown for facilitating protein function analysis. We also extend our studies on lipid-protein interactions to clarify the roles of lipid components in relation to viral pathogenicity. The results from our studies will not only assist in the development of novel drugs for WSSV and SGIV but may also serve as a paradigm for other important DNA viral pathogens. Currently, we are specifically concentrating on identification and characterization of virus non-structure proteins, DNA binding proteins, up/down regulated host proteins and lipids.

Selected Publications

1. Wu J, Lin Q, Lim TK, Liu T, Hew CL (2007) White spot syndrome virus proteins and differentially expressed cellular proteins identified in shrimp epithelium by shotgun proteomics and cleavable isotope-coded affinity tag. J. Virol. (in press)

2. Li Z, Lin Q, Chen J. Wu JL, Lim TK, Loh SS, Tang X, Hew CL (2007) Shotgun identification of Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes. Molecular and Cellular Proteomics (in press)

3. Leu JH, Chang CC, Wu JL, Hsu CW, Hirono I, Aoki T, Juan HF, Lo CF, Kou GH, Huang HC (2007)Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics, 8:120.

4. Tang X, Wu J, Sivaraman J and Hew CL (2007) Crystal Structure of Two Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus (WSSV) Shed Light on their Evolutionary Relationship. J. Vriol. 81:6709-6717.

5. Liu Y, Wu J, Song JX, Sivaraman J, Hew CL (2006) Identification of a novel nonstructural protein VP9 from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites. J. Vriol. 80:10419-27.

6. Lo, CF, Wu JL, Chang YS, Wang HC, Tsai JM, Kou GH (2004) Molecular characterization and pathogenicity of white spot syndrome virus. In: Current Trends in Bacterial & Viral Fish & Shrimp Diseases

7. Wu JL, Muroga K (2004) Apoptosis does not play an important role in the resistance of 'immune' Penaeus japonicus against white spot syndrome virus. J Fish Dis 27:15-21

8. Namikoshi A, Wu JL, Yamashita T, Nishizawa T, Nishioka T, Arimoto M, Muroga K (2004) Vaccination trials in Penaeus japonicus against white spot syndrome virus. Aquaculture 229:25-35

9. Wu JL, Nishioka T, Mori K, Nishizawa T, Muroga K (2002) A time-course study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol 13:391-403

10. Wu JL, Suzuki K, Arimoto M, Nishizawa T, Muroga K (2002) Preparation of an inoculum of white spot syndrome virus for challenge tests in Penaeus japonicus. Fish Pathol 37:65-70

11. Wu JL, Namikoshi A, Nishizawa T, Mushiake K, Teruya K, Muroga K (2001) Effects of shrimp density on transmission of penaeid acute viremia in Penaeus japonicus by cannibalism and the waterborne route. Dis Aquat Org 47:129-135