Department of Biological Science
National University of Singapore
14 Science Drive 4
Molecular Genetics (LSM1102)
General Biology (LSM1301)
Laboratory Techniques in Life Sciences (LSM2191)
Virology, Aquatic Viruses and Viral Diseases, Shrimp White Spot Syndrome Virus (WSSV), Fish Iridoviruses, SGIV
We are generally interested in aquatic viruses and viral diseases, while focus on two large DNA viruses: white spot syndrome virus (WSSV) and Singapore grouper iridovirus (SGIV). Using a high-throughput proteomics approach, we are able to analyze the viral and host cellular proteins. Through X-ray crystallography, NMR spectroscopy and cryo-electron microscopy, we are able to solve the structures of both viral proteins and viral particles. The results from our studies will not only assist in the development of novel drugs for WSSV and SGIV but may also serve as a paradigm for understanding viral morphogenesis.
Liu Y, Tran BN, Wang F, Ounjai P, Wu J, Hew CL. (2015) Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells. Sci. Rep. 5, 18696; doi: 10.1038/srep18696 (corresponding author)
Wang F, Liu Y, Zhu Y, Tran BN, Wu J, Hew CL. (2015) Singapore Grouper Iridovirus ORF75R is a Scaffold Protein Essential for Viral Assembly. Sci. Rep. 5, 13151; doi: 10.1038/srep13151 (corresponding author)
Wang L, Qiong QY, Wu J. (2013) A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus. Virus Res. 176:37-44. (corresponding author)
Tran BN, Chen L, Liu Y, Wu J, Velázquez Campoy A, Sivaraman J, Hew CL. (2011) A Novel Histone H3 Binding Protein ORF158L from the Singapore Grouper Iridovirus (SGIV). J Virol. 85(17): 9159–9166.
Liu Y., Wu J., Chen H., Hew CL, Yan J. (2010) DNA Condensates Organized by the Capsid Protein VP15 in White Spot Syndrome Virus. Virology 408:197-203. (co-first author)
Wu J, Chan R, Wenk MR, Hew CL (2010) Lipidomic study of intracellular Singapore grouper iridovirus (SGIV). Virology 399: 248-56.
Wu J, Lin Q, Lim TK, Liu T, Hew CL (2007) White spot syndrome virus proteins and differentially expressed cellular proteins identified in shrimp epithelium by shotgun proteomics and cleavable isotope-coded affinity tag. J. Virol. 81:11681-9.
Li Z, Lin Q, Chen J. Wu JL, Lim TK, Loh SS, Tang X, Hew CL (2007) Shotgun identification of Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes. Mol. Cell. Proteomic. 6:1609-20.
Leu JH, Chang CC, Wu JL, Hsu CW, Hirono I, Aoki T, Juan HF, Lo CF, Kou GH, Huang HC (2007)Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics, 8:120.
Tang X, Wu J, Sivaraman J and Hew CL (2007) Crystal Structure of Two Major Envelope Proteins VP26 and VP28 from White Spot Syndrome Virus (WSSV) Shed Light on their Evolutionary Relationship. J. Virol. 81:6709–6717.
Liu Y, Wu J, Song JX, Sivaraman J, Hew CL (2006) Identification of a novel nonstructural protein VP9 from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites. J. Virol. 80:10419-27.
Lo, CF, Wu JL, Chang YS, Wang HC, Tsai JM, Kou GH (2004) Molecular characterization and pathogenicity of white spot syndrome virus. In: Current Trends in Bacterial & Viral Fish & Shrimp Diseases
Wu JL, Muroga K (2004) Apoptosis does not play an important role in the resistance of ‘immune’ Penaeus japonicus against white spot syndrome virus. J. Fish Dis. 27:15-21.
Namikoshi A, Wu JL, Yamashita T, Nishizawa T, Nishioka T, Arimoto M, Muroga K (2004) Vaccination trials in Penaeus japonicus against white spot syndrome virus. Aquaculture 229:25-35.
Wu JL, Nishioka T, Mori K, Nishizawa T, Muroga K (2002) A time-course study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol. 13:391-403.
Wu JL, Suzuki K, Arimoto M, Nishizawa T, Muroga K (2002) Preparation of an inoculum of white spot syndrome virus for challenge tests in Penaeus japonicus. Fish Pathol. 37:65-70.
Wu JL, Namikoshi A, Nishizawa T, Mushiake K, Teruya K, Muroga K (2001) Effects of shrimp density on transmission of penaeid acute viremia in Penaeus japonicus by cannibalism and the waterborne route. Dis. Aquat. Org. 47:129-135.