CBIS Confocal: Nikon Biostation IM Q

Biostation IM Q (Plastic Model)

Nikon

The Nikon BioStation IM-Q is a compact cell incubator and monitoring system that allows users with minimal microscopy experience to conduct live cell imaging without a steep learning curve. 

Location: CBIS Confocal Microscopy Laboratory
Booked CBIS equipment before? Information for First Time Users

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About the Biostation IM Q

The BioStation IM-Q time-lapse imaging system facilitates a broad array of short-term and long-term time-lapse experiments, including studies of cell growth, morphology, and protein expression, by providing consistent environmental control of temperature, humidity and gas concentration in combination with phase and fluorescence imaging of exceptional quality.

Additionally, researchers can select from two types of microscope cameras. Perfusion capabilities further expand experimental possibilities.

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Features
  • Biostation-IM-C Chamber, for 3.5cm plastic petridish, comes with No focus drift design and a vibration damping chassis. Fully motorized XYZ
  • Illumination light source for phase contrast: Red LED Light
  • Epi-fluorescence observation: HG Precentered Fiber illuminator Intensilight C-HGFIE
  • Objective: 20X/0.5, 0.6 -2.0mm, with magnification of 10X, 20X and 40X
  • CO2 Gas mixer TK-0003MIGM-SN01, with built in humidifier (270ml) and temperature heater
  • Humidity control at 95% or higher, Temperature control at 37°C (+/- 0.1°C)
  • Epi-fluorescence observation: HG Precentered Fiber illuminator Intensilight C-HGFIE
  • Fluorescent filter: detailed as followings. However, the machine can accommodate only two filter cubes at one time

 

DAPI ET GFP ET mCherry ET YFP Blue GFP
Excitation Filters 340-380 450-490 540-580 490-510 379-401
Dichroic Mirrors 400 495 585 515 420
Barrier Filter 435-485 500-550 592-667 520LP 435-485

 

  • Magnification from 10X to 40X (air), NA 0.5
  • Built in cooled 1.3 Mega pixels Monochrome CCD Camera
  • BS-IM Perfusion Induction Unit
  • BS-IM Perfusion Medium Exchange Unit
  • Operation Software: Biostation IM. Multichannel, multipoint (stitching up to 6mm x 6mm), time lapse can be set up easily by following the instructions on the GUI.
  • Image format: .nex file created once the experiment starts. After capturing, a folder is created which stores the images as .ics and .ids files. Data are able to be exported to: JPG, TIFF, BMP, PNG and AVI
  • PC with Intel QuadCore Q8400 (2.66GHz /4M / 1333MHz), 4GB Ram, 22 inch LCD monitor, 1T hard disk, 1GB graphic card, DVD-RW and license window Vista.
  • Application: live cell fluorescent imaging