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Nikhil Kumar Tulsian

Research Fellow

Ph.D. (Biological Sciences)
National University of Singapore


M.Sc. (Biotechnology)
University of Hyderabad, India


B.Sc. (Microbiology, Genetics, Chemistry)
Osmania University, India

My project interests are in characterizing direct role of phosphodiesterase in the cyclic AMP-PKA signalling pathway. The ubiquitous second messenger cAMP, elicits multiple cellular pathways. Of which, its primary effector, protein kinase A, triggers numerous functions. Using in vitro (structural mass spectrometry) and in vivo (fluorescence spectroscopy, enzyme kinetics and cellular assays), I aim to understand how phosphodiesterase act on it to regulate the sensitivity and specificity of cAMP signal termination.

nikhil

Lim Xin Xiang

Research Fellow

Ph.D. (Biological Sciences)
National University of Singapore

B.Sc. (Life Science with Specialization in Molecular and Cell Biology)
National University of Singapore

Viral particles are composite macromolecular assemblies that are highly coordinated and primed to respond to a plethora of diverse vector/host perturbations throughout their lifecycle. I am particularly interested in deciphering how virus particles achieve metastability as they progress through their lifecycles. Consequently, the interactions of host antibodies are intricately coupled to the virus structure and dynamics in solution under host-specific environments. I aim to investigate how dynamics of viral capsids, particularly Dengue viruses, affects the mechanism of antibody action associated with virus neutralization by structural mass spectrometry.

xin xiang

Lim Xin Shan

Research Fellow

Ph.D. (Biological Sciences)
National University of Singapore

B.Sc. (Life Science with Specialization in Molecular and Cell Biology)
National University of Singapore

The function of a single biological molecule depends on the interplay between itself and different biological molecules within the cell. To perform its function within the cell the molecule must interact following the physical laws of thermodynamics and at the same time exhibiting specific spatial and temporal requirement of the cell. As such, the ability to identify biological molecules and their interactions are critical to the understanding of the complex processes within cells. My research interest lies in the characterization of interactions between different biological molecules such as proteins and lipids/membranes. The membrane house many receptors and is commonly the first site of interaction for any biological phenomenon. As such, I am particularly interested in characterization of how membranes regulate the function of membrane proteins and also how viral proteins interact with cell membranes during infection using mainly but not restricted to mass spectrometry.

xin shan

Abhijeet Ghode

Graduate Student

B.Tech. (Biological Sciences and Bioengineering)
IIT Kanpur, India

The termination of cAMP second messenger signalling is an important yet understudied phenomenon. Recent breakthroughs from our lab revealed direct extraction of cAMP bound to its primary receptor PKAR by phosphodiesterase enzyme via formation of a ternary complex. I aim to characterize key interactions within this complex orchestrating processive hydrolysis of cAMP using various tools such as HDX-MS, enzyme kinetics and fluorescence polarization. A number of mutations on these proteins have been uncovered recently that are linked to musculoskeletal disorders such as Acrodysostosis and Carney Complex. I also study such disease-linked mutations to obtain insights into the molecular mechanism of pathogenesis.

abhijeet

Ranita Ramesh

Graduate Student

B.E. (Biotechnology)
PES Institute of Technology, Bengaluru, India 

I work on whole viral dynamics in solution, specifically focusing on the Turnip Crinkle Virus. Host specific environmental perturbations are required for the virus to sense that it has entered into the host cell. Once the virus enters into the cell, it prepares to release its genomic cargo to produce more copies of itself. I currently study the changes in dynamic behavior of the virus to understand the mechanism with which it senses this change in environment, that would initiate viral disassembly.

ranita

Palur Venkata Raghuvamsi

Graduate Student

M.Tech. (Medical Biotechnology)
IIT Hyderabad, India


B.Tech. (Biotechnology)
SASTRA University, India

I'm fascinated by how molecular motions at the atomistic scale in biomolecules collectively give rise to complexities extending from single cell to organismal level. My major interests encompass structural biology and molecular biophysics. I'm intrigued by the versatility in protein function and the necessity to include dynamics before function in the “sequence structure-function” model to completely understand the functionality of biomolecules. Currently, I'm working on Dengue capsid protein interaction with its genome to decipher its assembly and disassembly process.

raghu

Yong Xin Ee

Graduate Student
(Co-supervised)

B.Sc. (Life Science)
National University of Singapore

Dengue fever affects more than 390 million people annually and still, an effective vaccine is unavailable. I am interested in studying the Dengue Virus life cycle and broadly neutralizing antibodies against the virus using biophysical techniques such as HDXMS, time-resolved Foster Resonance Energy Transfer and Fluorescence Correlation Spectroscopy.

xinee

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