Biophysical Fluorescence Laboratory
Center For Bioimaging Sciences
Departments of Biological Sciences & Chemistry

Imaging FCS in APEER


Introduction

APEER is a free cloud-based platform to offer customizable image processing task solutions to the microscopy community. We have created modules translated from our home-built ImagingFCS_1.52 readily combined to customized your Imaging FCS workflows either for processing individual image stack or image stack in batch. The workflows runs locally somewhere and it's not shared. We believe in the spirit of open-source and therefore by making the code available as modules on the APEER platform it could help users achieving their best results. Please refer to user manual for more detail information on how to Imaging FCS on APEER,

apeerProfile


APEER modules


List of modules available:

  • FCS
  • Dual Color-FCCS
  • Region of Interest Selector
  • Optospit Image Splitter
  • Bleach correction
  • Background subtraction
  • Z-channel/frame binning

    • APEER workflows

    1. FCS workflow performs autocorrelations of image stacks acquired from camera-based Fluorescence Correlation Spectroscopy (FCS). It calculate correlations, curve fitting and visualization. The module works with any 16-bit or 32-bit image stacks in any of the standard file formats: TIFF, OME-TIFF, TIF.

    Sample output: interactive.html & output.xlsx

    workflowfcs


    2. DC-FCCS workflow is a tool to measure molecular interactions. It is based on the the simulataneous observation of two spectrally separate fluorescence signals imaged on the two regions of the same camera. By cross-correlating the different signals of pixels at two different wavelength but corresponding to the same sample location one can determine whether the different molecules/particles interact. Analysing DC-FCCS measurements can be achieved either by Total Internal Reflection Fluorescence (TIRF) microscopy or Selective Plane Illumination Microscope (SPIM).

    Sample Output: interactive.html & output.xlsx

    workflowpreparedcfccs

    workflowdcfccs

    Last update: 14 July 2020 (Version 1)

    Disclaimer

    The software and data on this site are provided for personal or academic use only and may not be used in any commercial venture or distributions. All files have been virus scanned, however, for your own protection; you should scan these files again. You assume the entire risk related to your use of this software and data. By using the software and data on this site your expressly assume all risks of data loss or damage alleged to have been caused by the software and data. The Biophysical Fluorescence Laboratory at NUS is providing this data "as is," and disclaims any and all warranties, whether express or implied, including (without limitation) any implied warranties of merchantability or fitness for a particular purpose. In no event will the Biophysical Fluorescence Laboratory at NUS and/or NUS be liable to you or to any third party for any direct, indirect, incidental, consequential, special or exemplary damages or lost profit resulting from any use or misuse of this software and data.
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    • Biophysical Fluorescence Laboratory