Here we directly look at membrane dynamics which is used as a matric for the peptide induced membrane deformation. This approach helped us to decode a time and concentration dependent interaction of monomeric human islet amyloid polypeptide (hIAPP) with model and live cell membranes. In particular, we were able to show the temporal evolution of membrane dynamics after hIAPP addition as a time lapse ‘FCS movie’ which allows one to directly visualize concerted spatial change in membrane diffusion with pixel resolution. ‘FCS movies’, to the best of our knowledge, is the first demonstration of time lapse diffusion imaging. We are currently applying this method to assay the action of other peptides and other model AMPs.
hIAPP FCS movie
Bag, N; Ali, A; Chauhan, VS; Wohland, T; Mishra, A. Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy, Chem Commun (Camb). 49 (80) (2013) 9155-7.