2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 1995-2001
2024
[150] Athilingam T, Nelanuthala AVS, Breen C, Karedla N, Fritzsche M, Wohland T, Saunder TE. "Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo" Development. 2024. DOI: doi.org/10.1242/dev.202128.
2023
[149] Tang WH, Sim SR, Aik DYK, Nelanuthala AVS, Athilingam T, Röllin A, Wohland T. "Deep learning reduces data requirements and allows real-time measurements in Imaging FCS" Biophys J. 2023. DOI: doi.org/10.1016/j.bpj.2023.11.3403.
[148] Sankaran J, Wohland T. "Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications." Commun Biol. 2023. DOI: doi.org/10.1038/s42003-023-05069-6.
2022
[147] Balasubramanian H, Sankaran J, Pandey S, Goh CJH, Wohland T. "The dependence of EGFR oligomerization on environment and structure: A camera-based N&B study." Biophys J. 2022. DOI: doi.org/10.1016/j.bpj.2022.11.003.
[146] Tantirimudalige SN, Raghuvamsi PV, Sharma KK, Bao JCW, Anand GS, Wohland T. "The ganglioside GM1a functions as a coreceptor/attachment factor for dengue virus during infection." J Biol Chem.. 2022. DOI: doi.org/10.1016/j.jbc.2022.102570.
[145] Aik DYK, Wohland T. "Microscope alignment using real-time Imaging FCS." Biophys J. 2022. DOI: doi.org/10.1016/j.bpj.2022.06.009.
[144] Gupta A, Lu D, Balasubramanian H, Chi Z, Wohland T. "Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes." Journal of Lipid Research. 2022. DOI: doi.org/10.1016/j.jlr.2022.100220.
2021
[143] Lyu X, Wang J, Wang J, Yin YS, Zhu Y, Li LL, Huang S, Peng S, Xue B, Liao R, Wang SQ, Long M, Wohland T, Chua BT, Sun Y, Li P, Chen XW, Xu L, Chen FJ, Li P. "A gel-like condensation of Cidec generates lipid-permeable plates for lipid droplet fusion." Developmental Cell. 2021. DOI: doi.org/10.1016/j.devcel.2021.08.015.
[142] Koh A, Sarusie MV, Ohmer J, Fischer U, Winkler C, Wohland T. "Fluorescence Correlation Spectroscopy Reveals Survival Motor Neuron Oligomerization but No Active Transport in Motor Axons of a Zebrafish Model for Spinal Muscular Atrophy." Front Cell Dev Biol. 2021. DOI: 10.3389/fcell.2021.639904.
[141] Yong XE, Palur VR, Anand GS, Wohland T, Sharma KK. "Dengue virus 2 capsid protein chaperones the strand displacement of 5'-3' cyclization sequences." Nucleic Acids Res. 2021. DOI: 10.1093/nar/gkab379.
[140] Dhasmana D, Veerapathiran S, Azbazdar Y, Nelanuthala AVS, Teh C, Ozhan G, Wohland T. "Wnt3 Is Lipidated at Conserved Cysteine and Serine Residues in Zebrafish Neural Tissue." Front Cell Dev Biol. 2021. DOI: 10.3389/fcell.2021.671218.
[139] Sankaran J, Balasubramanian H, Tang WH, Ng XW, Röllin A, Wohland T. "Simultaneous Spatiotemporal Super-Resolution and Multi-Parametric Fluorescence Microscopy." Nat Commun. 2021. DOI: 10.1038/s41467-021-22002-9. Protocol for live cell preparation available Here.
[138] Hackl S, Ng XW, Lu D, Wohland T, Becker CFW."Cytoskeleton-dependent clustering of membrane-bound prion protein on the cell surface." J Biol Chem. 2021. DOI: 10.1016/j.jbc.2021.100359.
[137] Koh A, Tao S, Jing Goh Y, Chaganty V, See K, Purushothaman K, Orbán L, Mathuru AS, Wohland T, Winkler C."A Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish." Hum Mol Genet. 2021. DOI: 10.1093/hmg/ddaa260.
2020
[136] Yong XE, Raghuvamsi PV, Anand GS, Wohland T, Sharma KK."Dengue virus strain 2 capsid protein switches the annealing pathway and reduces the intrinsic dynamics of the conserved 5’ untranslated region." RNA Biology. 2020. DOI: 10.1101/2020.06.19.160978.
[135] Veerapathiran S, Teh C, Zhu S, Kartigayen I, Korzh V, Matsudaira PT, Wohland T. "Wnt3 distribution in the zebrafish brain is determined by expression, diffusion and multiple molecular interactions." eLife. 2020. DOI: 10.7554/eLife.59489.
[134] Vidaillac C, Yong VFL, Aschtgen MS, Qu J, Yang S, Xu G, Seng ZJ, Brown AC, Ali MK, Jaggi TK, Sankaran J, Foo YH, Righetti F, Nedumaran AM, Mac Aogáin M, Roizman D, Richard JA, Rogers TR, Toyofuku M, Luo D, Loh E, Wohland T, Czarny B, Horvat JC, Hansbro PM, Yang L, Li L, Normark S, Henriques Normark B, Chotirmall SH. "Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa." mBio. 2020. DOI: 10.1128/mBio.01774-20.
[133] Wohland T. "Splitting the Difference: Sorting Photons to Improve Quantitative Measurements in Correlation Spectroscopy." Biophys J. 2020. DOI: 10.1016/j.bpj.2020.08.017.
[132] Nielsen CD, Dhasmana D, Floresta G, Wohland T & Cilibrizzi A. "Illuminating the path to target GPCR structures and functions." Biochemistry. 2020. DOI: 10.1021/acs.biochem.0c00606.
[131] Gupta A, Phang IY, Wohland T. "To Hop or not to Hop: Exceptions in the FCS Diffusion Law." Biophys J. 2020. DOI: 10.1016/j.bpj.2020.04.004.
[130] Sankaran J, Wohland T. "Fluorescence strategies for mapping cell membrane dynamics and structures." APL Bioengineering. 2020. DOI: 10.1063/1.5143945.
[129] Gupta A, Muralidharan S, Torta F, Wenk MR, Wohland T. "Long acyl chain ceramides govern cholesterol and cytoskeleton dependence of membrane outer leaflet dynamics." BBA Biomembranes. 2020, 1862 (3), 183153-183153. DOI: 10.1016/j.bbamem.2019.183153.
[128] Gupta A, Korte T, Herrmann A, Wohland T. "Plasma membrane asymmetry of lipid organization: fluorescence lifetime microscopy and correlation spectroscopy analysis." J Lipid Res. 2020. DOI: 10.1194/jlr.D119000364.
2019
[127] Sankaran J, Tan NJHJ, But KP. et al. "Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms." NPJ Biofilms Microbiomes. 2019. DOI: 10.1038/s41522-019-0107-4.
[126] Azbazdar Y, Ozalp O, Sezgin E, Veerapathiran S, Duncan AL, Sansom MSP, Eggeling C, Wohland T, Karaca E, Ozhan G. "More Favorable Palmitic Acid Over Palmitoleic Acid Modification of Wnt3 Ensures Its Localization and Activity in Plasma Membrane Domains." Front Cell Dev Biol. 2019. DOI: 10.3389/fcell.2019.00281.
[125] Furlan A, M Gonzalez-Pisfil, A Leray, D Champelovier, M Henry, C Le Nézet, O Bensaude, M Lefranc, T Wohland, B Vandenbunder, G Bidaux, and L Héliot. "HEXIM1 Diffusion in the Nucleus Is Regulated by Its Interactions with Both 7SK and P-TEFb." Biophys J. 2019. DOI: 10.1016/j.bpj.2019.09.019.
[124] Grüter A, Hoffmann M, Müller R, Wohland T, Jung G. "A high-affinity fluorescence probe for copper(II) ions and its application in fluorescence lifetime correlation spectroscopy." Anal Bioanal Chem. 2019. DOI: 10.1007/s00216-019-01798-y.
[123] Zhao P, Teng X, Tantirimudalige SN, Nishikawa M, Wohland T, Toyama Y, Motegi F. "Aurora-A Breaks Symmetry in Contractile Actomyosin Networks Independently of Its Role in Centrosome Maturation." Dev Cell. 2019. DOI: 10.1016/j.devcel.2019.02.012.
[122] Gupta A, Marzinek JK, Jefferies D, Bond PJ, Harryson P, Wohland T. "The disordered plant dehydrin Lti30 protects the membrane during water-related stress by cross-linking lipids." J Biol Chem. 2019. DOI: 10.1074/jbc.RA118.007163.
[121] Sharma KK, Lim XX, Tantirimudalige SN, Gupta A, Marzinek JK, Holdbrook D, Lim XYE, Bond PJ, Anand GS, Wohland T. "Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations."Structure. 2019. DOI: 10.1016/j.str.2018.12.006.
2018
[120] Gupta A, Sankaran J, Wohland T. "Fluorescence correlation spectroscopy: The technique and its applications in soft matter." Physical Sciences Reviews. 2018. DOI: 10.1021/ja0508976.
[119] Ng XW, Sampath K, Wohland T. "Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish." Methods Mol Biol. 2018. DOI: 10.1007/978-1-4939-8772-6_5.
[118] Wang J, Yin Y, Lau S, Sankaran J, Rothenberg E, Wohland T, Meier-Schellersheim M, Knaut H. "Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs." Dev Cell. 2018. DOI: 10.1016/j.devcel.2018.07.015.
[117] Sharma KK, Marzinek JK, Tantirimudalige SN, Bond PJ, Wohland T. "Single-molecule studies of flavivirus envelope dynamics: Experiment and computation." Prog Biophys Mol Biol. 2018. DOI: 10.1016/j.pbiomolbio.2018.09.001.
[116] Veerapathiran S, Wohland T. "Fluorescence techniques in developmental biology." J Biosci. 2018. DOI: 10.1007/s12038-018-9768-z.
[115] Marzinek JK, Bag N, Huber RG, Holdbrook DA, Wohland T, Verma CS, Bond PJ. "A Funneled Conformational Landscape Governs Flavivirus Fusion Peptide Interaction with Lipid Membranes." J Chem Theory Comput. 2018 Jun 6. doi: 10.1021/acs.jctc.8b00438.
[114] Ng J, Kamm RD, Wohland T, Kraut RS. "Evidence from ITIR-FCS Diffusion Studies that the Amyloid-Beta (Aβ) Peptide Does Not Perturb Plasma Membrane Fluidity in Neuronal Cells." J Mol Biol. 2018. DOI: 10.1016/j.jmb.2018.04.030.
[113] Harwardt MIE, Dietz MS, Heilemann M, Wohland T. "SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules." Biophys J. 2018. DOI: 10.1016/j.bpj.2018.03.013.
[112] Sankaran J, Karampatzakis A, Rice SA, Wohland T. "Quantitative imaging and spectroscopic technologies for microbiology".FEMS Microbiol Lett. 2018. DOI: 10.1093/femsle/fny075.
[111] Hoischen C, Yavas S, Wohland T, Diekmann S. "CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres." PLoS One. 2018. DOI: 10.1371/journal.pone.0192572.
[110] Mistri TK, Arindrarto W, Ng WP, Wang C, Lim LH, Sun L, Chambers I, Wohland T, Robson P. "Dynamic changes in Sox2 spatio-temporal expression promote the second cell fate decision through Fgf4/Fgfr2 signaling in preimplantation mouse embryos." Biochem J. 2018. DOI: 10.1042/BCJ20170418.
2017
[109] Bag N., Huang S., Wohland T. (2017) "Investigating the Dynamics and Organization of Membrane Proteins and Lipids by Imaging Fluorescence Correlation Spectroscopy." In: Chattopadhyay A. (eds) Membrane Organization and Dynamics. Springer Series in Biophysics2017. DOI: 10.1007/978-3-319-66601-3_6.
[108] Veerapathiran S, Wohland T. "The imaging FCS diffusion law in the presence of multiple diffusive modes." Methods. 2017. DOI: 10.1016/j.ymeth.2017.11.016.
[107] Karampatzakis A, Song CZ, Allsopp LP, Filloux A, Rice SA, Cohen Y, Wohland T, Török P. "Probing the internal micromechanical properties of Pseudomonas aeruginosa biofilms by Brillouin imaging." NPJ Biofilms Microbiomes. 2017. DOI: 10.1038/s41522-017-0028-z.
[106] Niu L, Wohland T, Knoll W, Köper I. "Interaction of a synthetic
antimicrobial peptide with a model bilayer platform mimicking
bacterial membranes." Biointerphases. 2017. DOI:
10.1116/1.5001020.
[105] Huang S, Lim SY, Gupta A, Bag N, Wohland T. "Plasma membrane organization and dynamics is probe and cell line dependent". Biochim Biophys Acta. 2017. DOI: 10.1016/j.bbamem.2016.12.009.
[104] Ragunathan P, Sielaff H, Sundararaman L, Biuković G, Subramanian Manimekalai MS, Singh D, Kundu S, Wohland T, Frasch W, Dick T, Grüber G. "The uniqueness of subunit α of mycobacterial F-ATP synthases: An evolutionary variant for niche adaptation." J Biol Chem. 2017. DOI: 10.1074/jbc.M117.784959.
[103] Sezgin E, Azbazdar Y, Ng XW, Teh C, Simons K, Weidinger G, Wohland T, Eggeling C, Ozhan G. "Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments." FEBS J. 2017. DOI: 10.1111/febs.14139.
[102] Karampatzakis A, Sankaran J, Kandaswamy K, Rice SA, Cohen Y and Wohland T. "Measurement of oxygen concentrations in bacterial biofilms using transient state monitoring by single plane illumination microscopy." Biomed. Phys. Eng. Express 2017. DOI: 10.1088/2057-1976/aa6db7.
[101] Lim XX, Chandramohan A, Lim XY, Bag N, Sharma KK, Wirawan M, Wohland T, Lok SM, Anand GS. "Conformational changes in intact dengue virus reveal serotype-specific expansion." Nat Commun. 2017. DOI: 10.1038/ncomms14339.
2016
[100] Yavas S, Macháň R, Wohland T. "The Epidermal Growth Factor Receptor Forms Location-Dependent Complexes in Resting Cells." Biophys J. 2016. DOI: 10.1016/j.bpj.2016.09.049.
[99] Bag N, Ng XW, Sankaran J, Wohland T. "Spatiotemporal mapping of diffusion dynamics and organization in plasma membranes." Methods and Applications in Fluorescence. 2016. DOI: 10.1088/2050-6120/4/3/034003.
[98] Ng XW, Teh C, Korzh V, Wohland T. "The secreted signaling protein wnt3 is associated with membrane domains in vivo: a SPIM-FCS study." Biophys J. 2016. DOI: 10.1016/j.bpj.2016.06.021.
[97] Macháň R, Foo YH, Wohland T. "On the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements." Biophys J. 2016. DOI: 10.1016/j.bpj.2016.06.001.
[96] Wang Y, Wang X, Wohland T, Sampath K. "Extracellular interactions and ligand degradation shape the Nodal morphogen gradient." Elife. 2016. DOI: 10.7554/eLife.13879.
[95] Ge J, Zhang CW, Ng XW, Peng B, Pan S, Du S, Wang D, Li L, Lim KL, Wohland T, Yao SQ. "Puromycin Analogues Capable of Multiplexed Imaging and Profiling of Protein Synthesis and Dynamics in Live Cells and Neurons." Angew Chem Int Ed Engl. 2016. DOI: 10.1002/anie.201511030.
2015
[94] Krieger, J.W; Singh, A.P; Bag, N; Garbe, C.S; Saunders, T.E; Langowski, J; Wohland, T. "Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms." Nat Protoc. 2015. DOI: 10.1038/nprot.2015.100.
[93] Bag, N; Huang, S; Wohland, T. "Plasma Membrane Organization of Epidermal Growth Factor Receptor in Resting and Ligand-Bound States." Biophys J. 2015. DOI: 10.1016/j.bpj.2015.09.007.
[92] Eshaghi, M; Sun, G; Grüter, A; Lim, C.L; Chee, Y.C; Jung, G; Jauch, R; Wohland, T; Chen, S. L. "Rational Structure-Based Design of Bright GFP-Based Complexes with Tunable Dimerization." Angew Chem Int Ed Engl. 2015. DOI: 10.1002/anie.201506686.
[91] Teh C, Sun G, Shen H, Korzh V, Wohland T. "Modulating expression level of secreted Wnt3 influences cerebellum development in zebrafish transgenics." Development. 2015. DOI: 10.1242/dev.127589.
[90] Mistri, T.K.; Devasia, A.G.; Chu, L.T.; Ng, W.P.; Halbritter, F.; Colby, D.; Martynoga, B.; Tomlinson, S.R.; Chambers, I.; Robson, P.; Wohland, T. "Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells." EMBO Rep 2015. DOI: 10.15252/embr.201540467.
[89] Wohland, T. "Single molecule data under scrutiny: Comment on “Extracting physics of life at the molecular level: A review of single-molecule data analyses by W. Colomb & SK Sarkar." Phys Life Rev 2015. DOI: 10.1016/j.plrev.2015.01.017.
[88] Ng, X.W.; Bag, N.; Wohland, T. "Characterization of Lipid and Cell Membrane Organization by the Fluorescence Correlation Spectroscopy Diffusion Law." CHIMIA International Journal for Chemistry2015. DOI: 10.2533/chimia.2015.112
[87] Rashid, R.; Chee, S.M.L.; Raghunath, M.; Wohland, T. "Macromolecular Crowding Gives Rise to Microviscosity, Anomalous Diffusion & Accelerated Actin Polymerization." Physical Biology2015. DOI: 10.1088/1478-3975/12/3/034001
[86] Sun, G.; Guo, S.M.; Teh, .; Korzh, V,; Bathe, M.; Wohland, T. "Bayesian Model Selection Applied to the Analysis of FCS Data of Fluorescent Proteins in vitro and in vivo." Analytical chemistry2015. DOI: 10.1021/acs.analchem.5b00022
2014
[85] Rashid, R.; Lim, N.S.J.; Chee, S.M.L.; Png, S.N.; Wohland, T.; Raghunath, M. "Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation, Tissue Engineering Part C" Methods2014. DOI: 10.1089/ten.TEC.2013.0733.
[84] Wohland, T. "Scan and Conquer: A Novel Approach to the Analysis of Interactions by Molecular Brightness Determination." Biophys. J. 2014. DOI: 10.1016/j.bpj.2014.04.056.
[83] Rashid, R.; Beyer, S.; Blocki, A.; Le Visage, C.; Trau, D.; Wohland, T.; Raghunath, M. "Mitochondrial Routing of Glucose and Sucrose Polymers After Pinocytotic Uptake: Avenues For Drug Delivery." Biomacromolecules2014. DOI: 10.1021/bm500243m.
[82] Machan, R.; Wohland, T. "Recent Applications of Fluorescence Correlation Spectroscopy in Live Systems." FEBS Letters2014. DOI: 10.1016/j.febslet.2014.03.056.
[81] Singh, A.P.; Wohland, T. "Applications of imaging fluorescence correlation spectroscopy." Curr. Op. Chem. Biol.2014. DOI: 10.1016/j.cbpa.2014.04.006.
[80] Guo, S.M.; Bag, N.; Mishra, A.;Wohland, T.; Bathe, M. "Bayesian Total Internal Reflection Fluorescence Correlation Spectroscopy Reveals hIAPP-Induced Plasma Membrane Domain Organization in Live Cells." Biophy. J.2014. DOI: 10.1016/j.bpj.2013.11.4458.
[79] Bag, N.; Yap, D.H.X.; Wohland, T. "Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy." BBA Biomembranes2014. DOI: 10.1016/j.bbamem.2013.10.009.
[78] Krieger, J.W.; Singh, A.P.; Garbe, C.S.; Wohland, T.; Langowski, J. "Dual-Color Fluorescence Cross-Correlation Spectroscopy on a Single Plane Illumination Microscope (SPIM-FCCS)." Optics Express. 2014. DOI: 10.1364/OE.22.002358. [Supplementary].
[77] Bag, N.; Wohland, T. "Imaging Fluorescence Fluctuation Spectroscopy: New Tools for Quantitative Bioimaging." Annu. Rev. Phys. Chem.2014. DOI: 10.1146/annurev-physchem-040513-103641.[76] Bag, N; Ali, A; Chauhan, VS; Wohland, T; Mishra, A. "Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy." Chem Commun2013. DOI: 10.1039/c3cc44880k.
[75] Sankaran, J.; Bag, N.; Kraut, R.S.; Wohland, T. "Accuracy and precision in camera-based fluorescence correlation spectroscopy measurements." Anal. Chem.2013. DOI: 10.1021/ac303485t.
[74] Singh, A.P.; Krieger, J.W.; Buchholz, J.; Charbon, E.; Langowski, J.; Wohland, T. "The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy." Optics Express.2013. DOI: 10.1364/OE.21.008652. [Supplementary]
2012
[73] Lauterbach, T.; Manna, M.; Ruhnow, M.; Wisantoso, Y.; Wang, Y.; Matysik, A.; Oglecka, K.; Mu, Y.; Geifman-Shochat, S.; Wohland, T.; Kraut, R. "Weak Glycolipid Binding of a Microdomain-Tracer Peptide Correlates with Aggregation and Slow Diffusion on Cell Membranes." Plos ONE2012. DOI: 10.1371/journal.pone.0051222.
[72] Lam, C.S.; Mistri, T.K.; Foo, Y.H.; Sudhaharan, T.; Gan, H.T.; Rodda, D.; Lim, L.H.; Chou, C.; Robson, P.; Wohland, T.; Ahmed, S. "DNA dependent Oct4-Sox2 interaction and diffusion properties characteristics of the pluripotent cell state revealed by fluorescence spectroscopy." Biochem J.2012. DOI: 10.1042/BJ20120725.
[71] Bag, N.; Sankaran, J.; Paul, A.; Kraut, R.; Wohland, T. "Calibration and Limits of Camera-Based Fluorescence Correlation Spectroscopy: A Supported Lipid Bilayer Study." Chemphyschem2012. DOI: 10.1002/cphc.201200032.
[70] Kay, J.G.; Koivusalo, M.; Ma, X.; Wohland, T.; Grinstein, S. "Phosphatidylserine dynamics in cellular membranes." Mol Bio Cell2012. DOI: 10.1091/mbc.E11-11-0936.
[69] Guo, S-M.; He, J.; Monnier, N.; Sun, G.; Wohland, T.; Bathe, M. "A Bayesian approach to the analysis of fluorescence correlation spectroscopy data II: Application to simulated and in vitro data." Anal Chem2012. DOI: 10.1021/ac2034369.
[68] Foo, Y.H.; Naredi-Rainer, N.; Lamb, D.C.; Ahmed, S.; Wohland, T. "Factors Affecting the Quantification of Biomolecular Interactions by Fluorescence Cross-Correlation Spectroscopy." Biophys J2012. DOI: 10.1016/j.bpj.2012.01.040.
[67] Korzh, V.; Wohland, T. "Analysis of properties of single molecules in vivo or... why small fish is better than empty dish." Russian Journal of Developmental Biology2012. DOI: 10.1007/s00424-013-1243-y.
[66] Kraut, R.; Bag, N.; Wohland, T. "Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes." Methods in Cell Biology2012. DOI: 10.1016/B978-0-12-386487-1.00018-3.
2011
[65] Ma, X.; Ahmed, S.; Wohland, T. "EGFR activation monitored by SW-FCCS in live cells." Front Biosci (Elite Ed)2011. DOI: 10.2741/e216.
[64] Broboana, D.; Mihai Balan, C.; Wohland,T.; Balan, C. "Investigations of the unsteady diffusion process in microchannels." Chemical Engineering Science2011. DOI: 10.1016/j.ces.2011.01.048.
[63] Zhou, L.; Liu, S.P.; Chen, L.Y.; Li, J.; Ong, L.B.; Guo, l.; Wohland, T.; Tang, C.C.; Lakshminarayanan, R.; Mavinahalli, J.; Verma, C.; Beuerman, R.W. "The structural parameters for antimicrobial activity, human epithelial cell cytotoxicity and killing mechanism of synthetic monomer and dimer analogues derived from hBD3 C-terminal region." Amino Acids2011. DOI: 10.1007/s00726-010-0565-8.
2010
[62] Wohland, T.; Shi, X.; Sankaran, J.; Stelzer, E.H. "Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments." Opt Express2010. DOI: 10.1364/OE.18.010627.
[61] Wang, X.; Wohland, T.; Korzh, V. "Developing in vivo biophysics by fishing for single molecules." Dev Biol2010. DOI: 10.1016/j.ydbio.2010.08.004.
[60] Sankaran, J.; Shi, X.; Ho, L.Y.; Stelzer, E.H.; Wohland, T. "ImFCS: a software for imaging FCS data analysis and visualization." Opt Express. 2010. DOI: 10.1364/OE.18.025468. [Supplementary]
[59] Lin, L.; Beyer, S.; Wohland, T.; Trau, D.; Lubrich, D. "Surface-bound microenclosures for biomolecules." Angew Chem Int Ed Engl2010. DOI: 10.1002/anie.200907321.
[58] Leptihn, S.; Har, J.Y.; Wohland, T.; Ding, J.L. "Correlation of Charge, Hydrophobicity, and Structure with Antimicrobial Activity of S1 and MIRIAM Peptides." Biochemistry2010. DOI: 10.1021/bi1011578.
[57] Ang, P.K.; Jaiswal, M.; Lim, C.H.; Wang, Y.; Sankaran, J.; Li, A.; Lim, C.T.; Wohland, T.; Barbaros O.; Loh K.P. "A bioelectronic platform using a graphene-lipid bilayer interface." ACS Nano2010. DOI: 10.1021/nn1022582.
2009
[56] Zhang, D.; Manna, M.; Wohland, T.; Kraut, R. "Alternate raft pathways cooperate to mediate slow diffusion and efficient uptake of a sphingolipid tracer to degradative and recycling compartments." J Cell Sci2009. DOI: 10.1194/jlr.m700543-jlr200.
[55] Yu, L.; Guo, L.; Ding, J.L.; Ho, B.; Feng, S.S.; Popplewell, J.; Swann, M.; Wohland, T. "Interaction of an artificial antimicrobial peptide with lipid membranes." Biochim Biophys Acta2009. DOI: 10.1016/j.bbamem.2008.10.005.
[54] Sudhaharan, T.; Liu, P.; Foo, Y.H.; Bu, W.; Lim, K.B.; Wohland, T.; Ahmed S. "Determination of in vivo dissociation constant, KD, of Cdc42-effector complexes in live mammalian cells using single wavelength fluorescence cross-correlation spectroscopy." J Biol Chem. 2009. DOI: 10.1074/jbc.M900894200.
[53] Shi, X.; Teo, L.S.; Pan, X.; Chong, S.W.; Kraut, R.; Korzh, V.; Wohland, T. "Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy." Dev Dyn. 2009. DOI: 10.1002/dvdy.22140.
[52] Shi, X.; Foo, Y.H.; Sudhaharan, T.; Chong, S.W.; Korzh, V.; Ahmed, S.; Wohland, T. "Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy." Biophys J. 2009. DOI: 10.1016/j.bpj.2009.05.006.
[51] Sankaran, J.; Manna, M.; Guo, L.; Kraut, R.; Wohland, T. "Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy." Biophys J. 2009. DOI: 10.1016/j.bpj.2009.08.025.
[50] Pan, X.; Shi, X.; Korzh, V.; Yu, H.; Wohland, T. "Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements." J Biomed Opt. 2009. DOI: 10.1117/1.3094947.
[49] Olaru, A.; Gheorghiu, M.; David, S.; Wohland, T.; Gheorghiu, E. "Assessment of the Multiphase Interaction between a Membrane Disrupting Peptide and a Lipid Membrane." J Phys Chem B. 2009. DOI: 10.1021/jp905170u.
[48] Liu, J.; Saw, C.L.; Olivo, M.; Sudhaharan, T.; Ahmed, S.; Heng, P.W.; Wohland, T. "Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques." J Biomed Opt. 2009. DOI: 10.1117/1.3067726.
[47] Leptihn, S.; Har, J.Y.; Chen, J.; Ho, B.; Wohland, T.; Ding, J.L. "Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria." BMC Biol. 2009. DOI: 10.1186/1741-7007-7-22.
[46] Heyder, J.; Beck-Speier, T.; Ferron, G.A.; Josten, M.; Karg, E.; Kreyling, W.G.; Lenz, A.G.; Maier, K.L.; Reitmeier, P.; Ruprecht, L.; Takenaka, S.; Wohland, T.; Ziesenis, A.; Schulz, H. "Long-term responses of canine lungs to acidic particles." Inhal Toxicol. 2009. DOI: 10.1080/08958370802651994.
[45] Dobkin-Bekman, M.; Naidich, M.; Rahamim, L.; Przedecki, F.; Almog, T.; Lim, S.; Melamed, P.; Liu, P.; Wohland, T.; Yao, Z.; Seger, R.; Naor, Z. "A preformed signaling complex mediates GnRH-activated ERK phosphorylation of paxillin and FAK at focal adhesions in L beta T2 gonadotrope cells." Mol Endocrinol. 2009. DOI: 10.1210/me.2008-0260.
[44] Ang, P.K.; Loh, K.P.; Wohland, T.; Nesladek, M.; Van Hove, E. "Supported Lipid Bilayer on Nanocrystalline Diamond: Dual Optical and Field-Effect Sensor for Membrane Disruption." Adv Funct Mater. 2009. DOI: 10.1002/adfm.200800770.
2008
[43] Yu, L.; Ding, J.L.; Ho, B.; Feng, S.S.; Wohland, T.; "Investigation of the Mechanisms of Antimicrobial Peptides Interacting with Membranes by Fluorescence Correlation Spectroscopy." The Open Chemical Physics Journal. 2008. DOI: 10.2174/1874412500801010062.
[42] Yeon, W.C.; Kannan, B.; Wohland, T.; Ng, V. "Colloidal crystals from surface-tension-assisted self-assembly: a novel matrix for single-molecule experiments." Langmuir. 2008. DOI: 10.1021/la800016h.
[41] Liu, P.; Ahmed, S.; Wohland, T. "The F-techniques: advances in receptor protein studies." Trends Endocrinol Metab. 2008. DOI: 10.1016/j.tem.2008.02.004.
[40] Korzh, S.; Pan, X.; Garcia-Lecea, M.; Winata, C.L.; Wohland, T.; Korzh, V.; Gong, Z. "Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish." BMC Dev Biol. 2008. DOI: 10.1186/1471-213X-8-84.
[39] Hebbar, S.; Lee, M.; Manna, M.; Steinert, S.; Kumar, G.S.; Wenk, M.; Wohland, T.; Kraut, R. "A fluorescent sphingolipid binding domain peptide probe interacts with sphingolipids and cholesterol-dependent raft domains." J Lipid Res. 2008. DOI: 10.1194/jlr.M700543-JLR200.
[38] Guo, L.; Har, J.Y.; Sankaran, J.; Hong, Y.M.; Kannan, B.; Wohland, T. "Molecular diffusion measurement in lipid Bilayers over wide concentration ranges: A comparative study." Chemphyschem. 2008. DOI: 10.1002/cphc.200700611.
[37] Du, Y.; Han, R.; Wen, F.; Ng San San, S.; Xia, L.; Wohland, T.; Leo, H.L.; Yu, H. "Synthetic sandwich culture of 3D hepatocyte monolayer." Biomaterials. 2008. DOI: 10.1016/j.biomaterials.2007.09.016.
[36] Chammika, N.B.U.; Chan, S.F.; Sureerat, H.; Bettiol, A.A.; Wohland, T.; Watt, F. "Fabrication of integrated channel waveguides in polydimethylsiloxane (PDMS) using proton beam writing (PBW): applications for fluorescence detection in microfluidic channels." Proceedings of Spie - The International Society for Optical Engineering. 2008. DOI: 10.1117/12.762885.
2007
[35] Wang, L.P.; Shao, P.G.; van Kan J.A.; Ansari, K.; Bettiol, A.A.; Pan, X.T.; Wohland, T.; Watt, F. "Fabrication of nanofluidic devices utilizing proton beam writing and thermal bonding techniques." Nucl Instrum Meth B. 2007. DOI: 10.1016/j.nimb.2007.02.062.
[34] Saw, C.L.L.; Olivo, M.; Wohland, T.; Fu, C.Y.; Kho, K.W.; Soo, K.C.; Heng, P.W.S. "Effects of n-methyl pyrrolidone on the uptake of hypericin in human bladder carcinoma and co-staining with DAPI investigated by confocal microscopy." Technol Cancer Res T. 2007. DOI: 10.1177/153303460700600503.
[33] Pan, X.T.; Foo, W.; Lim, W.; Fok, M.H.Y.; Liu, P.; Yu, H.; Maruyama, I.; Wohland, T. "Multifunctional fluorescence correlation microscope for intracellular and microfluidic measurements." Review of Scientific Instruments. 2007. DOI: 10.1063/1.2740053.
[32] Pan, X.; Yu, H.; Shi, X.; Korzh, V.; Wohland, T. "Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy." J Biomed Opt. 2007. DOI: 10.1117/1.2435173.
[31] Maung, N.L.; Wohland, T.; Hsu, C.Y.S. "Enamel diffusion modulated by Er : YAG laser (Part 2). Organic matrix." Journal of Dentistry. 2007. DOI: 10.1016/j.jdent.2007.07.012.
[30] Maung, N.L.; Wohland, T.; Hsu, C.Y.S. "Enamel diffusion modulated by Er : YAG laser (Part 1) - FRAP." Journal of Dentistry. 2007. DOI: 10.1016/j.jdent.2007.07.0.11.
[29] Liu, P.; Sudhaharan, T.; Koh, R.M.L.; Hwang, L.C.; Ahmed, S.; Maruyama, I.N.; Wohland T. "Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy." Biophysical Journal. 2007. DOI: 10.1529/biophysj.106.102087.
[28] Kannan, B.; Guo, L.; Sudhaharan, T.; Ahmed, S.; Maruyama, I.; Wohland, T. "Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera." Anal Chem. 2007. DOI: 10.1021/ac0624546.
[27] Hwang, L.C.; Wohland, T. "Recent advances in fluorescence cross-correlation spectroscopy." Cell Biochem Biophys. 2007. DOI: 10.1016/s0959-440x(02)00368-8.
[26] Du, Y.N.; Han, R.B.; Ng, S.; Ni, J.; Sun, W.X.; Wohland, T.; Ong, S.H.; Kuleshova, L.; Yu, H. "Identification and characterization of a novel prespheroid 3-dimensional hepatocyte monolayer on galactosylated substratum." Tissue Engineering. 2007. DOI: 10.1089/ten.2006.0381.
2006
[25] Yu, L.L.; Tan, M.Y.; Ho, B.; Ding, J.L.; Wohland, T. "Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide." Analytica Chimica Acta. 2006. DOI: 10.1016/j.aca.2005.09.008.
[24] Pan, X.; Aw, C.; Du, Y.; Yu, H.; Wohland, T. "Characterization of poly(acrylic acid) diffusion dynamics on the grafted surface of 5poly(ethylene terephthalate) films by fluorescence correlation spectroscopy." Biophys. Rev. 2006. DOI: 10.1142/S1793048006000264.
[23] Li, P.; Sun, M.; Wohland, T.; Yang, D.W.; Ho, B.; Ding, J.L. "Molecular mechanisms that govern the specificity of sushi peptides for gram-negative bacterial membrane lipids." Biochemistry. 2006. DOI: 10.1021/bi0602765.
[22] Li, P.; Sun, M.; Wohland, T.; Ho, B.; Ding, J.L. "The molecular mechanism of interaction between sushi peptide and Pseudomonas endotoxin." Cell Mol Immunol. 2006. PMID: 16549045.
[21] Kannan, B.; Har, J.Y.; Liu, P.; Maruyama, I.; Ding, J.L.; Wohland, T. "Electron multiplying charge-coupled device camera based fluorescence correlation spectroscopy." Anal Chem. 2006. DOI: 10.1021/ac0600959.
[20] Hwang, L.C.; Leutenegger, M.; Gosch, M.; Lasser, T.; Rigler, P.; Meier, W.; Wohland, T. "Prism-based multicolor fluorescence correlation spectrometer." Opt Lett. 2006. DOI: 10.1364/ol.31.001310.
[19] Hwang, L.C.; Gosch, M.; Lasser, T.; Wohland, T.; "Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation." Biophys J. 2006. DOI: 10.1529/biophysj.105.074120.
2005
[18] Yu, L.; Ding, J.L.; Ho, B.; Wohland T. "Investigation of a novel artificial antimicrobial peptide by fluorescence correlation spectroscopy: an amphipathic cationic pattern is sufficient for selective binding to bacterial type membranes and antimicrobial activity." Biochim Biophys Acta. 2005. DOI: 10.1016/j.bbamem.2005.08.005.
[17] Hwang, L.C.; Wohland, T. "Single wavelength excitation fluorescence cross-correlation spectroscopy with spectrally similar fluorophores: resolution for binding studies." J Chem Phys. 2005. DOI: 10.1063/1.1862614.
2004
[16] Yu, L.; Bow, H.; Ding, J.L.; Wohland T. "Measuring the binding of an antimicrobial peptide with LPS by Fluorescence Correlation Spectroscopy." J. Teknologi. 2004. DOI: 10.11113/jt.v41.722.
[15] Li, P.; Wohland, T.; Ho, B.; Ding, J.L. "Perturbation of lipopolysaccharide (LPS) micelles by Sushi 3 (S3) antimicrobial peptide - The importance of an intermolecular disulfide bond in S3 dimer for binding, disruption, and neutralization of LPS." Journal of Biological Chemistry. 2004. DOI: 10.1074/jbc.M405606200.
[14] Hwang, L.C.; Wohland, T. "Dual-color fluorescence cross-correlation spectroscopy using single laser wavelength excitation." Chemphyschem. 2004. DOI: 10.1002/cphc.200301057.
2003
[13] Pick, H.; Preuss, A.K.; Mayer, M.; Wohland, T.; Hovius, R.; Vogel, H.; "Monitoring expression and clustering of the ionotropic 5HT3 receptor in plasma membranes of live biological cells." Biochemistry. 2003. DOI: 10.1021/bi026576d.
[12] Milon, S.; Hovius, R.; Vogel, H.; Wohland, T. "Factors influencing fluorescence correlation spectroscopy measurements on membranes: simulations and experiments." Chem Phys. 2003. DOI: 10.1016/S0301-0104(03)00018-1.
2002
[11] Whelan, R.J.; Wohland, T.; Neumann, L.; Huang, B.; Kobilka, B.K.; Zare, R.N. "Analysis of bimolecular interactions using a miniaturized surface plasmon resonance sensor." Analytical Chemistry. 2002. DOI: 10.1021/ac025669y.
[10] Simpson, G.J.; Wohland, T.; Zare, R.N.; "Irradiation of dye-doped microspheres with a stronqly focused laser beam results in alignment upon optical trapping." Nano Lett. 2002. DOI: 10.1021/nl015632d.
[9] Neumann, L.; Wohland, T.; Whelan, R.J.; Zare, R.N.; Kobilka, B.K. "Functional immobilization of a ligand-activated G-protein-coupled receptor." Chembiochem. 2002. DOI: 10.1002/1439-7633(20021004)3:10<993::AID-CBIC993>3.0.CO;2-Y.
[8] Hwang, L.C.; Wohland, T. "Fluorescence Correlation Spectroscopy for the Characterization of Membranes: A Short Review." Songkhlanakarin J. Sci. Technol. 2002. DOI: 10.1016/j.bbamem.2008.08.013.
1995-2001
[7] Wohland, T.; Rigler, R.; Vogel, H. "The standard deviation in fluorescence correlation spectroscopy." Biophys J. 2001. DOI: 10.1016/S0006-3495(01)76264-9.
[6] Vallotton, P.; Tairi, A.P.; Wohland, T.; Friedrich-Benet, K.; Pick, H.; Hovius, R.; Vogel, H. "Mapping the antagonist binding site of the serotonin type 3 receptor by fluorescence resonance energy transfer." Biochemistry. 2001. DOI: 10.1021/bi0106989.
[5] Hovius, R.; Vallotton, P.; Wohland, T.; Vogel, H. "Fluorescence techniques: shedding light on ligand-receptor interactions." Trends Pharmacol Sci. 2000. DOI: 10.1016/S0165-6147(00)01503-0.
[4] Wohland, T.; Friedrich, K.; Hovius, R.; Vogel H. "Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: evidence that the homopentameric 5-hydroxytryptamine type 3As receptor binds only one ligand." Biochemistry. 1999. DOI: 10.1021/bi990366s.
[3] Meseth, U.; Wohland, T.; Rigler, R.; Vogel H. "Resolution of fluorescence correlation measurements." Biophys J. 1999. DOI: 10.1016/S0006-3495(99)77321-2.
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