Trypanosoma brucei/sample prep by Cynthia He

🔹 Pre-Training

Step 1

• Unsure which instrument suits your research? Email Core staff to:
  • Discuss your project
  • Arrange a sample test on recommended instruments
• If you know the instrument needed, proceed to Step 2.

Step 2

• Apply for a user account on PPMS for the NUS_CBIS Facility

Step 3

• Prepare the following documents:
  • OSHE Certificate: CANVAS, Laser Safety training (OSHRAD02)
  • Experiment-Based Risk Assessment Form (Excel, example of RA for using confocals)
  • N3 License Application (PDF) – required for Abberior STEDYCON, 3i diSPIM, and TIRF1.

Step 4

• Log into PPMS and navigate to the Request tab
• Complete and submit the correct form:
  • CBIS LM_Widefield Imaging System User Registration Form: for widefield fluorescence, dark field, phase contrast, DIC microscopy
  • Laser Microscopy User Registration Form: for confocal, spinning disc, light sheet microscopy (upload OSHE certificate before submission)
  • User Registration Form for CBIS Cryostat 1850
• Email the completed Risk Assessment form to Core staff
• Facility staff will contact you to schedule training

Step 5

• Complete pre-training tutorials
• Prepare samples for training

🔹 Training

Part I – Day 1 (2 hours)

• Hands-on training by facility staff:
  • Standard imaging protocols
  • Data export
• Practice with your own samples under supervision

Part II – Day 2 (1 hour)

• Reinforce skills from Day 1
• Perform an independent imaging experiment
• Learn advanced image optimisation techniques

🔹 Post-Training

• Receive a post-training email with:
  • Details to join the Confocal User Contact Group
• Upon validation, you will gain:
  • Door access to trained systems
  • Booking rights for confocal systems via PPMS

Note: CBIS reserves the right to revoke user access for:

• Breaching facility regulations
• Damaging instruments
• Late or unpaid fees

Log into PPMS account at least once every three months to avoid deactivation.